Never been to an MBAA meeting? Join us at one of the following events. Normally our meetings are on the third Thursday of the month in January, February, March, April, October and November - our program year. Our regular meetings begin at 5pm with a Social Hour. For members, the cost for the meeting is $30 for the entire event, including dinner. See meeting for additional details.
February 2020 Monthly Meeting
February 20, 2020
5:00 PM - 8:00 PM
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Please Join Us for our February Technical Meeting as we welcome Lance Shaner, Founder/Owner, Omega Yeast.
His presentation is titled, Improved Functional Assays and Risk Assessment for diastatic (STA+) Saccharomyces cerevisiae.
Diastatic yeasts are a major contamination risk to packaged beer. Brewery quality control programs rely on microbial selective methods and PCR detection of the STA1 gene, however there is a wide functional range of diastatic activity that remains unresolved by these current methods. Herein, we provide a comprehensive analysis of diastatic yeast selection media using our collection of STA1+ strains. We highlight several factors to control including plate age, autoclave cycling conditions, cupric sulfate and dipotassium phosphate concentrations. We further present several functional assays that confirm the variation in diastatic activity across STA1+ strains and show a clear correlation with earlier onset and increasing strength in strains that contain an intact STA1 promoter. Furthermore, we have developed robust and simple plate-based assay to confirm diastatic activity of potential contaminants. Collectively, these functional assays provide improved risk assessment of re-fermentation in packaged product with the potential for determining contaminant thresholds for different STA1+ strains and weighing in on the decision to dump beer or issue a product recall.
$30.00 Member Registration (Includes Dinner)
$20.00 Member Registration (Without Dinner)
$60.00 Guest Registration